The CAP-e assay is performed by first incubating red blood cells (erythrocytes) with a test sample at a range of concentrations.
The cells are then combined with dichloro fluorescein diacetate (DCFDA), which is oxidized in the presence of free radicals to form a green fluorescent byproduct (DCF).
In the next step of the assay, exogenous hydrogen peroxide is added at a concentration of 167 mM to artificially induce severe oxidative stress.
The antioxidant activity of varying concentrations of the test compound is measured based on the degree of inhibition of DCF-fluorescence, which is an indirect and nonspecific measure of reactive oxygen species production.
To date, the assay has been used in 2 published studies, both conducted by AIBMR and Holger NIS.
The following chart shows the results of eXfuze products as well as certain other dietary supplements. These scientific CAP-e test results were obtained from an independent nationally recognized laboratory.
The CAP-e value is stated in Gallic Acid Equivalent (GAE) units. This measurement reflects the relative antioxidant protection of cells by the test product per weight, compared to the known antioxidant Gallic Acid. The higher the CAP-e value, the higher the relative antioxidant protection of cells.
The CAP-e assay is used to test whether natural products contain antioxidants capable of protecting live cells from oxidative damage. Thus, when any protective effect is seen in the CAP-e assay, it shows a biologically meaningful antioxidant protection by the product. The laboratory test results of the other dietary supplements were 3,1.2, and N/A, which meant that it was not achieved therefore could not be calculated.
Our conclusion is that these test results suggest the antioxidant ingredients in eXfuze products are able to penetrate living cells in the human body and protect the cells against oxidative stress.
eXfuze believes that this is compelling evidence that their products are some of the most effective products available in the marketplace to reduce oxidative stress.